Consensus Protein Localization:

The mean image is generated by interpolating the single cell histories onto a reference cell cycle.Time is measured relative to the cell cycle length and position is measured relative to the cell geometry. The std image is the standard deviation in intensity. The error image is the predicted error in the mean intesnity. The mean, std, and error images share a common normalization: intensity relative to the brightest pixel. The cell histories are individually corrected for protein synthesis, degredation and fluorophore bleahcing by adjust the temporal normalization to maintain a fixed areal mean intensity throughout the cell cycle.

Mean Intensity

N_Cell = 1000

Std Intensity

N_Cell = 1000

Error Intensity

N_Cell = 1000

Principal Component Analysis (Cell-to-Cell Variation):

Principal Component Analysis of Cell-to-Cell Variation captures the dominant modes of cell to cell variation.

Comp: 1

Power: 1

Comp: 2

Power: 0.47

Comp: 3

Power: 0.39

Comp: 4

Power: 0.32

Comp: 5

Power: 0.28

Comp: 6

Power: 0.24

Cell Splits: Subpopulation Analysis

To investigate the role of cell physiological parameter P (eg cell length) on protein localization, cells are divided into two subpopulations P_cell > P_cut and P_cell > P_cut and consensus images are computed for each. On the left hand side, each image is normalized individually. On right hand side, a common normalization scheme is used for all twelve subpopulation consensus images. On the far right, a histogram is shown for each parameter P for all complete cell cycles collected. The blue (red) curve shows the histogram for the population below (above) P_cut. The yellow curve shows the histogram generated by all fusions strains (typically 48) imaged in the imaging run.

N_Cell = 473

N_Cell = 527

N_Cell = 473

N_Cell = 527

N_Cell = 429

N_Cell = 571

N_Cell = 429

N_Cell = 571

N_Cell = 550

N_Cell = 450

N_Cell = 550

N_Cell = 450

N_Cell = 388

N_Cell = 612

N_Cell = 388

N_Cell = 612

N_Cell = 812

N_Cell = 188

N_Cell = 812

N_Cell = 188

N_Cell = 434

N_Cell = 566

N_Cell = 434

N_Cell = 566