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W3110 Unique ID: JW0579

Gene: fepE

Gene name: fepE
Strain: JW0579
Induction: 50 uM IPTG
Comment: 3B12C
Reference: PMID:16769691--ASKA Collection--Kitagawa et al.

Consensus Protein Localization:

The mean image is generated by interpolating the single cell histories onto a reference cell cycle.Time is measured relative to the cell cycle length and position is measured relative to the cell geometry. The std image is the standard deviation in intensity. The error image is the predicted error in the mean intesnity. The mean, std, and error images share a common normalization: intensity relative to the brightest pixel. The cell histories are individually corrected for protein synthesis, degredation and fluorophore bleahcing by adjust the temporal normalization to maintain a fixed areal mean intensity throughout the cell cycle.

Mean Intensity

NCell = 666

Std Intensity

NCell = 666

Error Intensity

NCell = 666

Cell Orientation Relative Intensity

Principal Component Analysis (Cell-to-Cell Variation):

Principal Component Analysis of Cell-to-Cell Variation captures the dominant modes of cell to cell variation.

Comp: 1

Power: 1

Comp: 2

Power: 0.49

Comp: 3

Power: 0.29

Comp: 4

Power: 0.24

Comp: 5

Power: 0.19

Comp: 6

Power: 0.18

Cell Splits: Subpopulation Analysis

To investigate the role of cell physiological parameter P (eg cell length) on protein localization, cells are divided into two subpopulations Pcell > Pcut and Pcell > Pcut and consensus images are computed for each. On the left hand side, each image is normalized individually. On right hand side, a common normalization scheme is used for all twelve subpopulation consensus images. On the far right, a histogram is shown for each parameter P for all complete cell cycles collected. The blue (red) curve shows the histogram for the population below (above) Pcut. The yellow curve shows the histogram generated by all fusions strains (typically 48) imaged in the imaging run.

Individual Normalization
Common Normalization
< Pcut
> Pcut
Characteristic P
< Pcut
> Pcut
Histograms

NCell = 299

NCell = 367

Cell Length

Lcut = 2.3 um

NCell = 299

NCell = 367

NCell = 226

NCell = 440

Cell-Cycle Length

Tcut = 77.1 min

NCell = 226

NCell = 440

NCell = 393

NCell = 273

Cell Birth Time

Tcut = 217.8 min

NCell = 393

NCell = 273

NCell = 0

NCell = 0

Number of

Neighbors

Ncut = NaN cells

NCell = 0

NCell = 0

NCell = 508

NCell = 158

Pole Age

Tcut = 2.3 divisions

NCell = 508

NCell = 158

NCell = 276

NCell = 390

Cell Fluor

Icut = 172.1 AU

NCell = 276

NCell = 390