Consensus Protein Localization:

The mean image is generated by interpolating the single cell histories onto a reference cell cycle.Time is measured relative to the cell cycle length and position is measured relative to the cell geometry. The std image is the standard deviation in intensity. The error image is the predicted error in the mean intesnity. The mean, std, and error images share a common normalization: intensity relative to the brightest pixel. The cell histories are individually corrected for protein synthesis, degredation and fluorophore bleahcing by adjust the temporal normalization to maintain a fixed areal mean intensity throughout the cell cycle.

Mean Intensity

N_Cell = 1000

Std Intensity

N_Cell = 1000

Error Intensity

N_Cell = 1000

Principal Component Analysis (Cell-to-Cell Variation):

Principal Component Analysis of Cell-to-Cell Variation captures the dominant modes of cell to cell variation.

Comp: 1

Power: 1

Comp: 2

Power: 0.85

Comp: 3

Power: 0.77

Comp: 4

Power: 0.71

Comp: 5

Power: 0.67

Comp: 6

Power: 0.64

Cell Splits: Subpopulation Analysis

To investigate the role of cell physiological parameter P (eg cell length) on protein localization, cells are divided into two subpopulations P_cell > P_cut and P_cell > P_cut and consensus images are computed for each. On the left hand side, each image is normalized individually. On right hand side, a common normalization scheme is used for all twelve subpopulation consensus images. On the far right, a histogram is shown for each parameter P for all complete cell cycles collected. The blue (red) curve shows the histogram for the population below (above) P_cut. The yellow curve shows the histogram generated by all fusions strains (typically 48) imaged in the imaging run.

N_Cell = 399

N_Cell = 601

N_Cell = 399

N_Cell = 601

N_Cell = 315

N_Cell = 685

N_Cell = 315

N_Cell = 685

N_Cell = 518

N_Cell = 482

N_Cell = 518

N_Cell = 482

N_Cell = 0

N_Cell = 0

N_Cell = 0

N_Cell = 0

N_Cell = 771

N_Cell = 229

N_Cell = 771

N_Cell = 229

N_Cell = 510

N_Cell = 490

N_Cell = 510

N_Cell = 490